Mouse 5-hydroxytryptamine ELISA Kit
SKU: 17176405007

Mouse 5-hydroxytryptamine ELISA Kit

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Description

Mouse 5-hydroxytryptamine ELISA KitThe Mouse 5 hydroxytryptamine ELISA Kit measures 5 HT in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 5 HT 5 hydroxytryptamine. 5 HT 5 hydroxytryptamine in the sample or standard competes with a fixed amount of biotin labeled 5 HT 5 hydroxytryptamine for sites on a pre coated antibody

The Mouse 5-hydroxytryptamine ELISA Kit measures 5-HT in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 5-HT/5-hydroxytryptamine. 5-HT/5-hydroxytryptamine in the sample or standard competes with a fixed amount of biotin-labeled 5-HT/5-hydroxytryptamine for sites on a pre-coated antibody specific to 5-HT/5-hydroxytryptamine. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 5-HT/5-hydroxytryptamine in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 17176405007

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