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Description
Beta-2 Microglobulin ELISAThe Beta 2 microglobulin ELISA is an enzyme immunoassay for the quantitative determination of Beta 2 microglobulin in human serum, plasma, and urine. The microtiter plate is coated with the antibody specifically binding the Beta 2 microglobulin. The human serum, plasma or urine is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with HRP labelled detection antibody. Unbound
The Beta 2-microglobulin ELISA is an enzyme immunoassay for the quantitative determination of Beta 2-microglobulin in human serum, plasma, and urine.
The microtiter plate is coated with the antibody specifically binding the Beta 2-microglobulin. The human serum, plasma or urine is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with HRP-labelled detection antibody. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of the Beta 2-microglobulin the specimen. The concentration of Beta 2-miroglobulin in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
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