SKU: 36554431577

Retinol Binding Protein 4 (RBP4) ELISA

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Description

Retinol Binding Protein 4 (RBP4) ELISAThe Retinol Binding Protein 4 (RBP4) ELISA is an enzyme immunoassay for the quantitative determination of Retinol Binding Protein 4 (RBP4) in human serum and plasma. The microtiter plate is coated with the antibody specifically binding the Retinol binding Protein 4. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin labelled detection

The Retinol-Binding Protein 4 (RBP4) ELISA is an enzyme immunoassay for the quantitative determination of Retinol-Binding Protein 4 (RBP4) in human serum and plasma.

The microtiter plate is coated with the antibody specifically binding the Retinol-binding Protein 4. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of RBP4 in the specimen. The concentration of RBP4 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

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SKU: 36554431577

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JoanieV.
Houston, US
★★★★★ 3
Great ball but easily broken
Size: Large
I got this ball for my two dogs, one is a 90 pound yellow lab mix and the other 45 pound black lab mix. So far only my bigger dog has played with it and has also busted it. He was playing with it for probably half an hour just chomping down to make it crinkle and crunch. It's quite the loud ball!! I noticed the sound wasn't as loud so I took a peek and noticed my dog basically crushed the inner ball into a much smaller ball. He's still having a good time with it though. It's only a matter of time though that he's going to chew the outer shell because it doesn't make noise like it did.
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Reviewed in the United States on December 31, 2024
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Mari Sned
Alexandria, US
★★★★★ 5
Dogs go for this ball every time
Size: Large, Size: Large
My Boston's and Rotti love this ball. I had to get two more. It's great for the Boston's because they can grasp it with the way it's made with the softer rubber. They love the crackle sound. It's just the right size and weight for smaller like the Boston Terrier and Rotti. Easy cleaning, rinses right off.
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Reviewed in the United States on May 13, 2026
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buckzmom
Port Orchard, US
★★★★★ 5
It's a Hit a Our House!
Size: Large
My strong chewer is still ..... Well, going strong! He loves his new crackle ball. He enjoys the new sound, the bounce back feeling in his jaws when chews. Mom is happy that pup hasn't put a dent in the toy yet! Price was more than reasonable too. We give it 5 Paws Up!
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Reviewed in the United States on May 25, 2026
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Lisa
Charlottesville, US
★★★★★ 4
Medium size preferred.
Size: Large
I purchased both the large 4" and the medium 3" balls. My 80 lbs German shepherd much prefers the medium size. Show loves to play fetch with it and its lasted well!
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Reviewed in the United States on May 10, 2026
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Sharon
Carnegie, US
★★★★★ 5
Chew toy that will last!
Color: Blue&Red Fish
Great toy for a real chewer. It's been in the house for about 5 days and the toy is still intact and the squeaker is still squeaking.
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Reviewed in the United States on May 28, 2026

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