SKU: 41703114468

Human IGFBP-2 (Insulin-like growth factor-binding protein 2) ELISA Kit

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Description

Human IGFBP-2 (Insulin-like growth factor-binding protein 2) ELISA KitThe Human IGFBP 2 (Insulin like growth factor binding protein 2) ELISA Kit measures IGFBP 2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IGFBP 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound

The Human IGFBP-2 (Insulin-like growth factor-binding protein 2) ELISA Kit measures IGFBP-2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IGFBP-2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IGFBP-2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IGFBP-2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 41703114468

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effie
Birmingham, US
★★★★★ 5
Squeaky
Size: 8, Color: Black
They look incredible. But they're a little squeaky. Otherwise my husband loves the comfort. They go with any outfit
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Reviewed in the United States on May 11, 2026
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Verified Purchase
Boito
Waukegan, US
★★★★★ 5
These fit very nicely, are comfortable, and work for my wide feet!
Size: 12 Wide, Color: Brown
Once I learned while shoe shopping in my youth that I needed wide-sized shoes, I wasn't able to easily choose from any shoes I saw in the store. I usually have to go find the wide widths and have to deal with the limited styles they have. Shopping online has been hit and miss, too. Sometimes, shoes are advertised as wide width, but end up still being too narrow. These shoes fit me just right. They were not too tight at the toes, gave good width at the arch, and the opening was large enough that I didn't have to undo every single lace just to get my foot in. They also look nice, and I'm sure will take a decent polish. Also, note that this does say it's an Oxford shoe, but it does have the open lacing and is more of a derby-style shoe. This also helps with sizing for my wide feet. If I didn't already have a pair of black shoes, I'd look at picking one of these up. My only reservation with these is that the soles are not dark-colored to complement a more formal style. I feel the white makes them a little more casual. The sole is also more transparent/opaque coloring at the heel and underneath, which you can kind of see-through, so keep that in mind. It's not fully clear, but is kind of transparent, which I was not expecting. I think it also shows where it picks up dirt as you wear it. Just more of a style cue one way or another. As a reference, I buy the 4E wide and Extra Wides in New Balance and Skechers. I also have a hard time getting any good fit in Nike shoes.
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Reviewed in the United States on October 2, 2023
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Michelle sealock
Lowell, US
★★★★★ 5
Looks and feel good
Size: 10.5, Color: Black
Shoes look dressy but feel comfortable. They are stiff but very comfortable.
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Reviewed in the United States on June 8, 2026
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Riley H.
Birmingham, US
★★★★★ 5
Well made and affordable
Size: 10.5, Color: Brown
Very comfortable, great price!!!!!!
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Reviewed in the United States on April 30, 2026
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George M. Occhipinti
Alexandria, US
★★★★★ 5
Very handsome shoe
Size: 7, Color: Brown
Beautifully made shoe. Comfortable, great fit. Very handsome on foot. Can wear with anything. Very pleased
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Reviewed in the United States on April 18, 2026

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