Rat Ang-II (Angiotensin II) ELISA Kit

Rat Ang-II (Angiotensin II) ELISA KitWith about 53 known citations, the Rat Ang II (Angiotensin II) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat Ang . During the reaction, Rat Ang in the sample or standard competes with a fixed amount of Rat Ang on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang . Excess conjugate and unbound sample or standard are washed away, and Avidin

With about 53 known citations, the Rat Ang-II (Angiotensin II) ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-â…¡. During the reaction, Rat Ang-â…¡ in the sample or standard competes with a fixed amount of Rat Ang-â…¡ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-â…¡. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-â…¡ in tested samples can be calculated by comparing the OD of the samples to the standard curve.