Human C5 (Complement C5) QuickTest ELISA Kit
SKU: 68403638980

Human C5 (Complement C5) QuickTest ELISA Kit

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Description

Human C5 (Complement C5) QuickTest ELISA KitThe Human C5 (Complement C5) QuickTest ELISA Kit measures C5 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual wells.

The Human C5 (Complement C5) QuickTest ELISA Kit measures C5 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains C5, a capture antibody-C5-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C5 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 68403638980

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Learn how to fight the best way!
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This book was so amazing. This book helped me rethink my praying techniques. I absolutely love this book. It’s perfect for any woman looking to improve her prayer life.
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It’s a good read but tells how to pray against demon warfare but nowhere does it tell how you( the reader) can be delivered!
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