Mouse TL ELISA Kit
SKU: 38416424942

Mouse TL ELISA Kit

$228.57 $253.97
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Description

Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is

Product Specification

Sensitivity 9.375ng/ml
Species Reactivity Mouse
Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value.
Synonym Mouse TC (Total cholesterol) ELISA Kit
Detection Type Competition Law
Composition
Name 9 6 T  match   set Save conditions after opening
ELISA Enzyme plate 8 Hole ×12 Strip Place the unused holes in a zippered aluminum foil bag and add desiccant and store sealed. Available at 2-8°C Save 1 Months; In -20°C Save 6 Months.
Lyophilized Standard 2 branch
Place the unused standard in the desiccant pack. Available at 2-8°C Save 1 Months; In -20°C Save 6 Months.
Concentrated biotin - Antibody (lyophilized)
1 branch
concentrate HRP- Streptavidin 100X
120uL
2-8°C( Protect from light )
TMB Chromogenic substrate
 1 Bottle 10ml
Purified water
 200ul
 2-8°C
 
 
Sample dilution
 1 Bottle 20ml
Antibody dilution
 1 Bottle 10ml
 SABC Diluent
 1 Bottle 10ml
Reaction stop solution
 1 Bottle 10ml
Concentrated wash 25X
 1 Bottle 30ml
Coating
5 Zhang
 
 
Instructions
1 Share
Note: The volume of liquid reagent supplied in the reagent bottle is slightly larger than the label indicates. Please use a pipette to measure accurately and dilute accordingly.
General Notes 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure.
2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture).
3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation.
4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds.
5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure.
6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation.
7. Do not reuse disposable tips to avoid cross-contamination.
8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature.
9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.
Instructions Serum, plasma, cell culture supernatant, cell lysate and other fluid samples
Storage Temp. Unopened kit, stored at 2-8 °C, shelf life for one year.
Test Range 15.625-1000ng/ml
Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
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SKU: 38416424942

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Looking for something to hel you save up space, this is the one✅️✅️✅️
Color: White, Size: 15.7in, Color: White, Size: 15.7in
Help me save so much space , my space looks better now, give me the perfect vibe. Assembly was easy , arrive on great condition, the material is durable, and stay stable.
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Great shelves!
Color: White, Size: 15.7in, Color: White, Size: 15.7in
Absolutely love these shelves. They were pretty easy to install. They’re also real spacious and sturdy. I used them for my son’s cute little belongings. Definitely recommend!
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Battle Creek, US
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Will buy again
Color: Walnut, Size: 2 Pack
Great color, hardwear was easy to install, quality great, very stable, all in great fit great size and love the look
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Good product at a reasonable price.
Color: Grey, Size: 2 Pack
The shelves were easy to install, are sturdy and look good.
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Pawtucket, US
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Highly recommend these shelves
Color: Black, Size: 2 Pack
Love these shelves! Easy to put up, stylish, well made and mounting hardware feels secure. Love the fine details of the included screws and anchors and small tabs to cover screws underneath.
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Reviewed in the United States on May 27, 2026

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