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Description
Human RAB37 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the Ras-related protein Rab-37 (RAB37). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Ras-related protein Rab-37 (RAB37) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ras-related protein Rab-37 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RAB37, also known as RAB37, is a member of the RAS oncogene family and is encoded by the RAB37 gene. Rab proteins are low-molecular-weight GTPases that are key regulators of vesicle trafficking. Reducing Rab37 levels by RNA interference results in impaired glucose-induced insulin secretion and a decrease in the number of granules tightly associated with the plasma membrane. It is a novel Rab GTPase specifically expressed in the MC-9 mast cell line and bone marrow mast cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 2426 reviews
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Product Reviews
★★★★★ 5
Surprisingly good!
Format: Kindle
I wasn’t sure I could forgive them and that’s always my issue with betrayal books - can I forgive the mmc, plural in this case, but the author was absolutely able to get me to that point. Yea I struggled with one them especially but overall I loved the book!! Followed the author immediately
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Reviewed in the United States on December 29, 2025
★★★★★ 4
Amazing read!!
Format: Kindle
Where to begin. I loved this book. The characters had a way of pulling you in and making you holding your breath at the same time. I absolutely loved the fmc. Eva has a way of being vulnerable but strong. She is unwilling to give up. The mmc’s are truly horrible at the beginning. With that being said, I still found myself rooting for them. Over time you could them changing and how even when they wanted to hate her they just couldn’t find it in them. A truly remarkable love came from a terrible beginning. Loved it!
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Reviewed in the United States on December 12, 2025
★★★★★ 3
Not bad but read better omegaverse
Format: Kindle
Mmm. I have feelings. Some not good. Some good. It was an ok read. I've been on a omegaverse kick for awhile now. Love me some groveling alphas but this wasn't it. Actually there wasn't enough groveling and the fmc gave in WAY too quickly.
They all have f'ed childhood. The alphas, Dorian, Rafe and Cade met at an orphanage that did unspeakable things to them until Dorian and Rafe aged out at 18 and had ti wait a few years until they could "adopt" Cade, who was 3 years younger than them. They all become successful entrepreneur of sort, I think. They created an app called HeatLink.
The fmc, Eva, was sold to the pack by her horrible, abusing father. She endured just as much hardship. She is relentless with escaping the pack but she always gave in when they got too near. Like she couldn't keep her legs closed. She hates them but gave in everytime. I found her kind of weak in that sense.
The overall plot was fine. Didn't leave me yearning to read the next page. I did clock one of the identity of someone quickly though. Don't want to spoil it. Again, I've read better omegaverse books. The one good thing about the book is one of the lines from Cade on page 218: ""Please Eva. I'm sorry." She staggered back and I followed her on my knees, dragging myself. I didn't care. I just wanted her to hold me."" I'll admit, I melted a little bit for Cade, even though he was probably the worse a-hole out of the bunch.
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Reviewed in the United States on January 9, 2026
★★★★★ 5
Good Groveling!
Format: Kindle
⭐️⭐️⭐️⭐️.5|🌶️🌶️🌶️🌶️.5
This was really good! Definitely a dark romance! The plot was really good and was done well. I enjoyed the character development but I did wish the FMC told her pack she loved them at the end.
Overall I would recommend this to readers who like bully romances and groveling! 💖
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Reviewed in the United States on February 24, 2026
★★★★★ 2
it went on too long…
Format: Kindle
At first it was a good read and I was really into it then it got to be a little to much with every page being more about how “slick” she was or about constant sex… I like spice don’t get me wrong but this was just a little too much after a while and the plot was lost. I also felt like the ending was weird and awkward it was just a good build up to fall flat
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Reviewed in the United States on May 23, 2026